All the Disney characters on this list have appeared physically in the extended cut of Once Upon a Studio, a celebration of Disney's 100th anniversary. These characters had either appeared in animated features, cartoon shorts or hybrid features made by Walt Disney Animation Studios. This version would feature a total of 547 characters, an increase from the 543 featured in the existing short.
Animated shorts
Oswald the Lucky Rabbit
Mickey Mouse and Friends
- Mickey Mouse (Mickey's Birthday Party design)
- Minnie Mouse (Mickey's Birthday Party design)
- Donald Duck (Mickey's Birthday Party design)
- Daisy Duck (Mr. Duck Steps Out design)
- Huey, Dewey, and Louie (1950s/DuckTales design)
- Goofy (Mickey's Birthday Party design)
- Pluto (1940s design)
- Pete (Officer Duck design)
- Chip and Dale (1950s design)
- Horace Horsecollar (Mickey's Birthday Party design)
- Clarabelle Cow (Mickey's Birthday Party design)
- Clara Cluck (Mickey's Birthday Party design)
- Spike the Bee (Inferior Decorator design)
- Humphrey the Bear (1950s design)
- J. Audubon Woodlore (1950s design)
- Scrooge McDuck (Mickey's Christmas Carol design)
- Ludwig Von Drake (An Adventure in Color design)
Silly Symphonies
The Skeleton Dance
- Skeletons
Three Little Pigs
Flowers and Trees
- Boy Tree
- Lady Tree
The Ugly Duckling
- The Ugly Duckling
Ferdinand the Bull
- Ferdinand
Lambert the Sheepish Lion
- Lambert (as an adult)
Susie the Little Blue Coupe
Ben and Me
The Small One
- Small One
Back to Never Land
- Robin Williams as a Lost Boy
John Henry
- John Henry
Prep & Landing
- Lanny
- Wayne
The Ballad of Nessie
Paperman
- George
- Lipstick Paper Airplane
Feast
- Winston
Inner Workings
- Paul
Us Again
- Art
- Dot
Animated features
Snow White and the Seven Dwarfs
- Snow White
- Doc
- Happy
- Grumpy
- Sleepy
- Sneezy
- Bashful
- Dopey
- The Prince
- The Evil Queen (in hag form)
Pinocchio
Fantasia and Fantasia 2000
- Yen Sid
- Three Magic Brooms
- Melinda
- Brudus
- Bacchus
- Jacchus
- Hyacinth Hippo
- Ben Ali Gator
- Madame Upanova
- Chernabog
- Duke
- Joe
- Flying John
- Rachel
- Yo Yo Flamingo
- Spring Sprite
Dumbo
Bambi
Saludos Amigos and The Three Caballeros
Make Mine Music
- Casey
- Peter
- Sasha
- Sonia
- Ivan
- Johnny Fedora
- Alice Blue Bonnet
Fun and Fancy Free
Melody Time
- Joe
- Jenny
- Johnny Appleseed
- Pecos Bill
- Slue-Foot Sue
- Widowmaker
The Adventures of Ichabod and Mr. Toad
- J. Thaddeus Toad
- Cyril Proudbottom
- Angus MacBadger
- Rat
- Mole
- Ichabod Crane
- Katrina Van Tassel
- Brom Bones
- The Headless Horseman
Cinderella
- Cinderella
- Prince Charming
- Jaq and Gus
- Two bluebirds
- Lady Tremaine
- Anastasia Tremaine
- Drizella Tremaine
- Lucifer
- Fairy Godmother
Alice in Wonderland
- Alice
- White Rabbit
- Tweedle Dee and Tweedle Dum
- Cheshire Cat
- Mad Hatter
- March Hare
- Queen of Hearts
- King of Hearts
Peter Pan
- Peter Pan
- Tinker Bell
- Wendy Darling
- John Darling
- Michael Darling
- Nana
- The Lost Boys
- Captain Hook
- Smee
- Tick-Tock the Crocodile
Lady and the Tramp
Sleeping Beauty
One Hundred and One Dalmatians
The Sword in the Stone
- Wart
- Merlin
- Archimedes
- Merlin's sugar bowl
- Madam Mim
Winnie the Pooh
The Jungle Book
- Mowgli
- Baloo
- Bagheera
- King Louie
- Colonel Hathi
- Winifred
- Hathi, Jr.
- Shere Khan
- Kaa
- Shanti
The Aristocats
Robin Hood
- Robin Hood
- Maid Marian
- Little John
- Lady Kluck
- Friar Tuck
- Alan-A-Dale
- Skippy
- Sis
- Tagalong
- Toby
- Prince John
- Sir Hiss
- Sheriff of Nottingham
The Rescuers and The Rescuers Down Under
- Bernard
- Miss Bianca
- Orville
- Penny
- Madame Medusa
- Mr. Snoops
- Cody
- Jake
- Percival C. McLeach
- Joanna
- Wilbur
The Fox and the Hound
The Black Cauldron
- Taran
- Eilonwy
- Fflewddur Fflam
- Gurgi
- Hen Wen
- Horned King
- Creeper
The Great Mouse Detective
Oliver & Company
The Little Mermaid
Beauty and the Beast
Aladdin
The Lion King
- Simba (as a cub)
- Nala (as a cub)
- Mufasa (as a spirit)
- Zazu
- Rafiki
- Timon
- Pumbaa
- Scar
- Shenzi, Banzai, and Ed
Pocahontas
The Hunchback of Notre Dame
Hercules
- Hercules
- Megara
- Pegasus
- Philoctetes
- Zeus
- Hades
- Pain and Panic
Mulan
Tarzan
Dinosaur
- Aladar
- Plio
- Yar
- Zini
- Suri
The Emperor's New Groove
Atlantis: The Lost Empire
- Milo Thatch
- Kida Nedakh
- Joshua Sweet
- Vincenzo Santorini
- Audrey Ramirez
- Cookie
- Gaetan Molière
- Wilhelmina Packard
Lilo & Stitch
Treasure Planet
Brother Bear
Home on the Range
Chicken Little
- Chicken Little
- Abby Mallard
- Runt of the Litter
- Fish Out of Water
Meet the Robinsons
- Lewis
- Wilbur Robinson
- Bowler Hat Guy
- DOR-15
- Carl the Robot
- Lucille Krunklehorn
Bolt
The Princess and the Frog
Tangled
Wreck-It Ralph and Ralph Breaks the Internet
- Ralph
- Vanellope von Schweetz
- Fix-It Felix Jr.
- Sergeant Calhoun
- Taffyta Muttonfudge
- King Candy
- Shank
- KnowsMore
- Yesss
Frozen and Frozen II
- Anna
- Elsa
- Olaf
- Kristoff
- Sven
- Hans
- Grand Pabbie
- Oaken
- Ryder
- Honeymaren
- Lieutenant Mattias
- Bruni
- Trolls
Big Hero 6
Zootopia
- Judy Hopps
- Nick Wilde
- Chief Bogo
- Clawhauser
- Flash
- Mr. Big
- Fru Fru
- Koslov
- Bellwether
- Gazelle
Moana
Raya and the Last Dragon
Encanto
- Mirabel Madrigal
- Alma Madrigal
- Bruno Madrigal
- Luisa Madrigal
- Isabela Madrigal
- Antonio Madrigal
- Julieta Madrigal
- Agustín Madrigal
- Dolores Madrigal
- Camilo Madrigal
- Pepa Madrigal
- Félix Madrigal
Strange World
- Ethan Clade
- Searcher Clade
- Meridian Clade
- Jaeger Clade
- Callisto Mal
- Legend
- Splat
Wish
Hybrid features
The Reluctant Dragon
- The Reluctant Dragon
Mary Poppins
Bedknobs and Broomsticks
- King Leonidas
- Secretary Bird