All the Disney characters on this list have appeared physically in the 2023 short film Once Upon a Studio, a celebration of the company's 100th anniversary. These characters had either appeared in animated features, cartoon shorts or hybrid features made by Walt Disney Animation Studios; according to Disney, 543 characters appear in the film.[1]
Animated shorts[]
Oswald the Lucky Rabbit[]
Mickey Mouse and Friends[]
- Mickey Mouse (Mickey's Birthday Party design)
- Minnie Mouse (Mickey's Birthday Party design)
- Donald Duck (Mickey's Birthday Party design)
- Daisy Duck (Mr. Duck Steps Out design)
- Huey, Dewey, and Louie (1950s/DuckTales design)
- Goofy (Mickey's Birthday Party design)
- Pluto (1940s design)
- Pete (Officer Duck design)
- Chip and Dale (1950s design)
- Horace Horsecollar (Mickey's Birthday Party design)
- Clarabelle Cow (Mickey's Birthday Party design)
- Clara Cluck (Mickey's Birthday Party design)
- Spike the Bee (Inferior Decorator design)
- Humphrey the Bear (1950s design)
- J. Audubon Woodlore (1950s design)
- Scrooge McDuck (Mickey's Christmas Carol design)
- Ludwig Von Drake (An Adventure in Color design)
Silly Symphonies[]
The Skeleton Dance[]
- Skeletons
Three Little Pigs[]
Flowers and Trees[]
- Boy Tree
- Lady Tree
The Ugly Duckling[]
- The Ugly Duckling
Ferdinand the Bull[]
- Ferdinand
Lambert the Sheepish Lion[]
- Lambert (as an adult)
Susie the Little Blue Coupe[]
Ben and Me[]
The Small One[]
- Small One
Back to Never Land[]
- Robin Williams as a Lost Boy
John Henry[]
- John Henry
Prep & Landing[]
- Lanny
- Wayne
The Ballad of Nessie[]
Paperman[]
- George
- Lipstick Paper Airplane
Feast[]
- Winston
Inner Workings[]
- Paul
Us Again[]
- Art
- Dot
Animated features[]
Snow White and the Seven Dwarfs[]
- Snow White
- Doc
- Happy
- Grumpy
- Sleepy
- Sneezy
- Bashful
- Dopey
- The Prince
- The Evil Queen (in hag form)
Pinocchio[]
Fantasia and Fantasia 2000[]
- Yen Sid
- Three Magic Brooms
- Melinda
- Brudus
- Bacchus
- Jacchus
- Hyacinth Hippo
- Ben Ali Gator
- Madame Upanova
- Chernabog
- Duke
- Joe
- Flying John
- Rachel
Dumbo[]
Bambi[]
Saludos Amigos and The Three Caballeros[]
Make Mine Music[]
- Casey
- Peter
- Sasha
- Sonia
- Ivan
- Johnny Fedora
- Alice Blue Bonnet
Fun and Fancy Free[]
Melody Time[]
- Joe
- Jenny
- Johnny Appleseed
- Pecos Bill
- Slue-Foot Sue
- Widowmaker
The Adventures of Ichabod and Mr. Toad[]
- J. Thaddeus Toad
- Cyril Proudbottom
- Angus MacBadger
- Rat
- Mole
- Ichabod Crane
- Katrina Van Tassel
- Brom Bones
- The Headless Horseman
Cinderella[]
- Cinderella
- Prince Charming
- Jaq and Gus
- Perla (credits only)
- Two bluebirds
- Lady Tremaine
- Anastasia Tremaine
- Drizella Tremaine
- Lucifer
- Fairy Godmother
Alice in Wonderland[]
- Alice
- White Rabbit
- Tweedle Dee and Tweedle Dum
- Cheshire Cat
- Mad Hatter
- March Hare
- Queen of Hearts
- King of Hearts
Peter Pan[]
- Peter Pan
- Tinker Bell
- Wendy Darling
- John Darling
- Michael Darling
- Nana
- The Lost Boys
- Captain Hook
- Smee
- Tick-Tock the Crocodile
Lady and the Tramp[]
Sleeping Beauty[]
One Hundred and One Dalmatians[]
The Sword in the Stone[]
- Wart
- Merlin
- Archimedes
- Merlin's sugar bowl
- Madam Mim
Winnie the Pooh[]
The Jungle Book[]
- Mowgli
- Baloo
- Bagheera
- King Louie
- Colonel Hathi
- Winifred
- Hathi, Jr.
- Shere Khan
- Kaa
- Shanti
The Aristocats[]
Robin Hood[]
- Robin Hood
- Maid Marian
- Little John
- Lady Kluck
- Friar Tuck
- Alan-A-Dale
- Skippy
- Sis
- Tagalong
- Toby
- Prince John
- Sir Hiss
- Sheriff of Nottingham
The Rescuers and The Rescuers Down Under[]
- Bernard
- Miss Bianca
- Orville
- Penny
- Madame Medusa
- Mr. Snoops
- Cody
- Jake
- Percival C. McLeach
- Joanna
- Wilbur
The Fox and the Hound[]
The Black Cauldron[]
- Taran
- Eilonwy
- Fflewddur Fflam
- Gurgi
- Hen Wen
- Horned King
- Creeper
The Great Mouse Detective[]
Oliver & Company[]
The Little Mermaid[]
Beauty and the Beast[]
Aladdin[]
The Lion King[]
Pocahontas[]
The Hunchback of Notre Dame[]
Hercules[]
- Hercules
- Megara
- Pegasus
- Philoctetes
- Zeus
- Hades
- Pain and Panic
Mulan[]
Tarzan[]
Dinosaur[]
- Aladar
- Plio
- Yar
- Zini
- Suri
The Emperor's New Groove[]
Atlantis: The Lost Empire[]
- Milo Thatch
- Kida Nedakh
- Joshua Sweet
- Vincenzo Santorini
- Audrey Ramirez
- Cookie
- Gaetan Molière
- Wilhelmina Packard
Lilo & Stitch[]
Treasure Planet[]
Brother Bear[]
Home on the Range[]
Chicken Little[]
- Chicken Little
- Abby Mallard
- Runt of the Litter
- Fish Out of Water
Meet the Robinsons[]
- Lewis
- Wilbur Robinson
- Bowler Hat Guy
- DOR-15
- Carl the Robot
- Lucille Krunklehorn
Bolt[]
The Princess and the Frog[]
Tangled[]
Wreck-It Ralph and Ralph Breaks the Internet[]
- Ralph
- Vanellope von Schweetz
- Fix-It Felix Jr.
- Sergeant Calhoun
- Taffyta Muttonfudge
- King Candy
- Shank
- KnowsMore
- Yesss
Frozen and Frozen II[]
- Anna
- Elsa
- Olaf
- Kristoff
- Sven
- Hans
- Grand Pabbie
- Oaken
- Ryder
- Honeymaren
- Lieutenant Mattias
- Bruni
- Trolls
Big Hero 6[]
Zootopia[]
- Judy Hopps
- Nick Wilde
- Chief Bogo
- Clawhauser
- Flash
- Mr. Big
- Fru Fru
- Koslov
- Bellwether
- Gazelle
Moana[]
Raya and the Last Dragon[]
Encanto[]
- Mirabel Madrigal
- Alma Madrigal
- Bruno Madrigal
- Luisa Madrigal
- Isabela Madrigal
- Antonio Madrigal
- Julieta Madrigal
- Agustín Madrigal
- Dolores Madrigal
- Camilo Madrigal
- Pepa Madrigal
- Félix Madrigal
- One of Bruno's rats (credits only)
Strange World[]
- Ethan Clade
- Searcher Clade
- Meridian Clade
- Jaeger Clade
- Callisto Mal
- Legend
- Splat
Wish[]
Hybrid features[]
The Reluctant Dragon[]
- The Reluctant Dragon
Mary Poppins[]
Bedknobs and Broomsticks[]
- King Leonidas
- Secretary Bird
Pete's Dragon[]
Deleted/Unused Characters[]
Who Framed Roger Rabbit[]
- Roger Rabbit (featured in storyboard for ending shot[2])
Fantasia 2000[]
- Yo Yo Flamingo (planned but left behind[2])
Far From the Tree[]
- Marie (featured in storyboard for ending shot; cut because of difficulty animating CG models over moving hand drawn Flowers and Trees trees under time constraints[2])
- Unidentified raccoon (presumably Papa rather than Baby Boy; featured in storyboard for ending shot; cut because of difficulty animating CG models over moving hand drawn Flowers and Trees trees under time constraints[2])